Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human IRF5 Detection Kit, 50,000 Assay Points
HTRF Human IRF5 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 50,000 Assay Points
HTRF Human Total PR Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Filter, 565 nm, 30 nm Bandwidth
Molecular Devices fluorescence filter with holder provides a 565 nm center wavelength and 30 nm bandwidth for fluorescence and luminescence measurements on SpectraMax iD series microplate readers.
- Center wavelength 565 nm.
- Bandwidth 30 nm (full width).
- Supplied with a holder compatible with microplate reader filter slots.
- Designed for fluorescence and luminescence assays.
Molecular Devices MICROPLATE READER SPECTRAMAXABS 100-240V
MICROPLATE READER SPECTRAMAXABS 100-240V.
Revvity Health Sciences Inc HTRF Human Total MET Detection Kit, 50,000 Assay Points
HTRF Human Total MET Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-MET (Thr1234/1235) Detection Kit, Control Lysate
HTRF Human Phospho-MET (Thr1234/1235) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human ApoB Detection Kit, 10,000 Assay Points
HTRF Human ApoB Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 10,000 Assay Points
Molecular Devices Emission Filter, Time-Resolved, 665 nm, 10 nm
Molecular Devices time-resolved acceptor emission filter supplied as a filter slide with holder. It provides a 665 nm center wavelength with a 10 nm bandwidth for sensitive acceptor emission detection in time-resolved fluorescence assays and is designed for use in compatible multi-mode microplate readers.
- Center wavelength 665 nm.
- Bandwidth 10 nm.
- Filter type time-resolved acceptor emission.
- Form factor filter slide with holder.
- Intended applications TR-FRET and HTRF assays.
- Compatible with multi-mode microplate readers that accept filter slides.
Molecular Devices SpectraDrop Cover Slides, 4 μL, 1.0 mm Path
Molecular Devices SpectraDrop Micro-Volume cover slide refills provide replacement cover slides for low-volume absorbance and fluorescence measurements. Each pack contains three 4 μL cover slides with a 1.0 mm path length, designed to restore and extend micro-volume workflows while minimizing sample consumption.
- Pack contains three 4 μL cover slides.
- Path length 1.0 mm.
- Enables measurements with sample volumes as low as 2 μL.
- Designed for micro-volume absorbance and fluorescence measurements.
- Helps reduce sample consumption and increase throughput.
Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm, Horizontal
This fluorescence polarization filter is specified for fluorescence polarization assays on compatible microplate readers. It has a 595 nm center wavelength with a 35 nm bandwidth and a polarized horizontal orientation, and is supplied with a holder for mounting in standard filter slides or modules.
- Center wavelength 595 nm.
- Bandwidth 35 nm.
- Polarization: horizontal.
- Includes holder for filter slide or module mounting.
- Intended for fluorescence polarization assays on compatible multi-mode microplate readers.
Molecular Devices Fluorescence Polarization Filter, 465 nm, 35 nm, Polarized
Fluorescence polarization filter set designed for microplate fluorescence assays. Centered at 465 nm with a 35 nm bandwidth, the set provides vertically and horizontally polarized filters and includes a holder for installation in compatible microplate readers.
- Designed for fluorescence polarization measurements on microplate readers.
- Center wavelength 465 nm with a 35 nm bandwidth.
- Includes vertically and horizontally polarized filters for polarization assays.
- Supplied as a set of two filters with a single holder for installation.
- Enables selective excitation and improved signal specificity in fluorescence assays.
