Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Ace Glass, Inc. 600ML VESSEL 600ML VESSEL
600mL vessel with a 48mm O.D. neck to fit a #50 Ace-Thred, for connection to an umbrella manifold. Vessel has sloped sides. Working capacity of vessel is about 1/2 of volume.
Molecular Devices Fluorescence Polarization Filter, 590 nm, 20 nm, Vertical
Molecular Devices fluorescence polarization filter designed for fluorescence polarization assays in compatible microplate readers. The filter has a 590 nm center wavelength, a 20 nm full-width bandwidth, and is supplied with a mounting holder for installation.
- 590 nm center wavelength, 20 nm bandwidth.
- Polarized vertical orientation.
- Supplied with mounting holder for installation.
- Optimized for fluorescence polarization measurements.
- Suitable for use in compatible microplate and ELISA readers.
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm, Vertical
Molecular Devices fluorescence polarization filter with holder designed for fluorescence polarization assays and FP-compatible detection on compatible multi-mode microplate readers. The filter is specified for a 635 nm center wavelength with a 35 nm bandwidth and is polarized to enable fluorescence polarization measurements.
- Designed for fluorescence polarization assays and FP detection modes.
- Center wavelength 635 nm with 35 nm bandwidth.
- Polarized to enable measurement of fluorescence anisotropy.
- Includes holder for installation into compatible filter assemblies.
- Intended as an accessory for multi-mode microplate readers that accept replaceable filters.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Vertical
The Molecular Devices fluorescence polarization filter (MPN 6590-0094) is a vertical polarization filter engineered for fluorescence polarization assays with a 485 nm center wavelength and a 20 nm optical bandwidth. It is intended as a replacement or accessory filter for compatible multi-mode microplate readers.
- Optimized for fluorescence polarization assays at a 485 nm center wavelength.
- 20 nm bandwidth provides narrow spectral selection and reduced crosstalk.
- Vertical polarization orientation for polarization-based measurements.
- Designed for installation in filter-slide assemblies of compatible microplate readers.
- Replacement filter for restoring or modifying detection channels.
Revvity Health Sciences Inc HTRF Human Androgen Receptor Detection Kit, Control Lysate
HTRF Human Androgen Receptor Detection Kit, Control Lysate
Revvity Health Sciences Inc Saw Tooth (Fine) Generator Probe, 20 x 195 mm
Saw Tooth (Fine) Generator Probe, 20 x 195 mm
Revvity Health Sciences Inc HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF MDM2 Binding Kit, 500 Assay Points
HTRF MDM2 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Androgen Receptor Detection Kit, 500 Assay Points
HTRF Human Androgen Receptor Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points